H2N2V1, Main, Exploration, bibRecord, 000565

Generation and Characterization of Monoclonal Antibodies Specific to Avian Influenza H5N1 Hemagglutinin Protein.

Identifieur interne : 000565 ( Main/Exploration ); précédent : 000564; suivant : 000566

Generation and Characterization of Monoclonal Antibodies Specific to Avian Influenza H5N1 Hemagglutinin Protein.

Auteurs : Ankita Malik [Inde] ; V Vamsee Aditya Mallajosyula [Inde] ; Nripendra Nath Mishra [Inde] ; Raghavan Varadarajan [Inde] ; Satish Kumar Gupta [Inde]

Source :

Monoclonal antibodies in immunodiagnosis and immunotherapy [ 2167-9436 ] ; 2015.

RBID : pubmed:26683184

Descripteurs français

English descriptors

KwdEn :
- Animals, Antibodies, Monoclonal (biosynthesis), Antibodies, Viral (biosynthesis), Antibody Specificity, Ascites (immunology), Cross Reactions, Female, Hemagglutination Inhibition Tests, Hemagglutinin Glycoproteins, Influenza Virus (administration & dosage), Hemagglutinin Glycoproteins, Influenza Virus (analysis), Hemagglutinin Glycoproteins, Influenza Virus (genetics), Hemagglutinin Glycoproteins, Influenza Virus (immunology), Humans, Influenza A Virus, H1N1 Subtype (chemistry), Influenza A Virus, H1N1 Subtype (immunology), Influenza A Virus, H3N2 Subtype (chemistry), Influenza A Virus, H3N2 Subtype (immunology), Influenza A Virus, H5N1 Subtype (chemistry), Influenza A Virus, H5N1 Subtype (immunology), Influenza A Virus, H7N9 Subtype (chemistry), Influenza A Virus, H7N9 Subtype (immunology), Kinetics, Mice, Mice, Inbred BALB C, Protein Binding, Recombinant Proteins (administration & dosage), Recombinant Proteins (analysis), Recombinant Proteins (genetics), Recombinant Proteins (immunology).
MESH :
- chemical , administration & dosage : Hemagglutinin Glycoproteins, Influenza Virus, Recombinant Proteins.
- chemical , analysis : Hemagglutinin Glycoproteins, Influenza Virus, Recombinant Proteins.
- chemical , biosynthesis : Antibodies, Monoclonal, Antibodies, Viral.
- chemical , genetics : Hemagglutinin Glycoproteins, Influenza Virus, Recombinant Proteins.
- chemistry : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H3N2 Subtype, Influenza A Virus, H5N1 Subtype, Influenza A Virus, H7N9 Subtype.
- immunology : Ascites, Hemagglutinin Glycoproteins, Influenza Virus, Influenza A Virus, H1N1 Subtype, Influenza A Virus, H3N2 Subtype, Influenza A Virus, H5N1 Subtype, Influenza A Virus, H7N9 Subtype, Recombinant Proteins.
- Animals, Antibody Specificity, Cross Reactions, Female, Hemagglutination Inhibition Tests, Humans, Kinetics, Mice, Mice, Inbred BALB C, Protein Binding.

Abstract

Highly pathogenic avian influenza (HPAI) H5N1 virus has in the past breached the species barrier from infected domestic poultry to humans in close contact. Although human-to-human transmission has previously not been reported, HPAI H5N1 virus has pandemic potential owing to gain of function mutation(s) and/or genetic reassortment with human influenza A viruses. Monoclonal antibodies (MAbs) have been used for diagnosis as well as specific therapeutic candidates in several disease conditions including viral infections in humans. In this study, we describe the preliminary characterization of four murine MAbs developed against recombinant hemagglutinin (rHA) protein of avian H5N1 A/turkey/Turkey/1/2005 virus that are either highly specific or broadly reactive against HA from other H5N1 subtype viruses, such as A/Hong Kong/213/03, A/Common magpie/Hong Kong/2256/2006, and A/Barheaded goose/Quinghai/14/2008. The antibody binding is specific to H5N1 HAs, as none of the antibodies bound H1N1, H2N2, H3N2, or B/Brisbane/60/2008 HAs. Out of the four MAbs, one of them (MA-7) also reacted weakly with the rHA protein of H7N9 A/Anhui/1/2013. All four MAbs bound H5 HA (A/turkey/Turkey/1/2005) with high affinity with an equilibrium dissociation constant (KD) ranging between 0.05 and 10.30 nM. One of the MAbs (MA-1) also showed hemagglutination inhibition activity (HI titer; 31.25 μg/mL) against the homologous A/turkey/Turkey/1/2005 H5N1 virus. These antibodies may be useful in developing diagnostic tools for detection of influenza H5N1 virus infection.

DOI: 10.1089/mab.2015.0047
PubMed: 26683184

Affiliations:

Inde

Le document en format XML

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<front><div type="abstract" xml:lang="en">Highly pathogenic avian influenza (HPAI) H5N1 virus has in the past breached the species barrier from infected domestic poultry to humans in close contact. Although human-to-human transmission has previously not been reported, HPAI H5N1 virus has pandemic potential owing to gain of function mutation(s) and/or genetic reassortment with human influenza A viruses. Monoclonal antibodies (MAbs) have been used for diagnosis as well as specific therapeutic candidates in several disease conditions including viral infections in humans. In this study, we describe the preliminary characterization of four murine MAbs developed against recombinant hemagglutinin (rHA) protein of avian H5N1 A/turkey/Turkey/1/2005 virus that are either highly specific or broadly reactive against HA from other H5N1 subtype viruses, such as A/Hong Kong/213/03, A/Common magpie/Hong Kong/2256/2006, and A/Barheaded goose/Quinghai/14/2008. The antibody binding is specific to H5N1 HAs, as none of the antibodies bound H1N1, H2N2, H3N2, or B/Brisbane/60/2008 HAs. Out of the four MAbs, one of them (MA-7) also reacted weakly with the rHA protein of H7N9 A/Anhui/1/2013. All four MAbs bound H5 HA (A/turkey/Turkey/1/2005) with high affinity with an equilibrium dissociation constant (KD) ranging between 0.05 and 10.30 nM. One of the MAbs (MA-1) also showed hemagglutination inhibition activity (HI titer; 31.25 μg/mL) against the homologous A/turkey/Turkey/1/2005 H5N1 virus. These antibodies may be useful in developing diagnostic tools for detection of influenza H5N1 virus infection.</div>
</front>
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Generation and Characterization of Monoclonal Antibodies Specific to Avian Influenza H5N1 Hemagglutinin Protein.

Generation and Characterization of Monoclonal Antibodies Specific to Avian Influenza H5N1 Hemagglutinin Protein.

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Abstract

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